First, please choose the number of probes you would like to get per gene : Get one probe per gene. Get up to 2 probes per gene. Get all probes for . Now, please choose one or more options that you would like to see in the report : Gene name Function Product Locus_tag Start position on gene sequence Distance 5' oligo to 3' gene Probe length Free energy of hybridization Enthalpy of hybrid Entropy of hybrid Probe Tm Possible cross-hybridization targets Probe sequence concatenated with : none nucleotides of 5' sequence (max 15) nucleotides of 3' sequence (max 15) sequence in 5' sequence in 3'