First, please choose the number of probes you would like to get per gene :
Get one probe per gene.
Get up to 2 probes per gene.
Get all probes for .
Now, please choose one or more options that you would like to see in the report :
Start position on gene sequence
Distance 5' oligo to 3' gene
Free energy of hybridization
Enthalpy of hybrid
Entropy of hybrid
Possible cross-hybridization targets
Probe sequence concatenated with :
nucleotides of 5' sequence (max 15)
nucleotides of 3' sequence (max 15)
sequence in 5'
sequence in 3'