Gene2Oligo report for sequence name

The average Tm used for this computation is 74.5

Hybridization Map:
(Upper (+) strand is given 5' - 3', lower (-) strand is 3' - 5'. Numbers are hybridization Tm ([DNA] = 1.0000000000000002E-6 M, [Na+] = 1.0 M))

ACCATGATTACGGATTCACTGGC-CGTCGTTTTACAACGTCGTG ACTGGGAAAACCCTGGC-GTTACCCAACTTAATCG TGGTACTAATGCCTAAGTGACCG GCAGCAAAATGTTGCAGCAC-TGACCCTTTTGGGACCG CAATGGGTTGAATTAGC 72.69 70.96 71.14 70.8 CCTTG CAGCACATCCCCCTTTCG-CCAGCTGGCGTAATAGCG AAGAGGCCCGCACCGA-TCGCCCTTCCCAACAGT T GGAAC-GTCGTGTAGGGGGAAAGC GGTCGACCGCATTATCGC-TTCTCCGGGCGTGGCT AGCGGGAAGGGTTGTCA-A 72.08 72.02 75.51 72.64 7 GCGCAGCCTGAATGG-CGAATGGCGCTTTGCC TGGTTTCCGGCACCAG-AAGCGGTGCCGGAAAG CTGGCTGGAGTGC CGCGTCGGACTTACC GCTTACCGCGAAACGG-ACCAAAGGCCGTGGTC TTCGCCACGGCCTTTC-GACCGACCTCACG 1.81 70.82 70.99 71.68 71.39 GATC-TTCCTGAGGCCGATACTGT CGTCGTCCCCTCAAACTGG-CAGATGCACGGTTACGATGC GCCCATCTACACCA CTAG AAGGACTCCGGCTATGACA-GCAGCAGGGGAGTTTGACC GTCTACGTGCCAATGCTACG-CGGGTAGATGTGGT 71.92 74.1 72.96 70.73 ACGTA-ACCTATCCCATTACGGTCAATC CGCCGTTTGTTCCCACG-GAGAATCCGACGGGTTGTTA CTCGCTCACATT TGCAT TGGATAGGGTAATGCCAGTTAG-GCGGCAAACAAGGGTGC CTCTTAGGCTGCCCAACAAT-GAGCGAGTGTAA 70.98 73.0 70.96 70.90 TAATGTTGATGA-AAGCTGGCTACAGGAAGGC CAGACGCGAATTATTTTTGATGGC-GTTAACTCGGCGTTTCATCTG ATTACAACTACT TTCGACCGATGTCCTTCCG-GTCTGCGCTTAATAAAAACTACCG CAATTGAGCCGCAAAGTAGAC- 73.82 72.6 70.73 TGGTGCAACGGGCGCT-GGGTCGGTTACGGCCA GGACAGTCGTTTGCCGT-CTGAATTTGACCTGAGCGCA TTTTTAC ACCACGTTGCCCGCGA CCCAGCCAATGCCGGT-CCTGTCAGCAAACGGCA GACTTAAACTGGACTCGCGT-AAAAATG 77.14 73.03 71.47 72.42 70.85 GCGCCGGAGA-AAACCGCCTCGCGGTG ATGGTGCTGCGTTGGA-GTGACGGCAGTTATCTGGA AGATCAGGATATGTG CGCGGCCTCT TTTGGCGGAGCGCCAC-TACCACGACGCAACCT CACTGCCGTCAATAGACCT-TCTAGTCCTATACAC 74.60 70.66 70.68 72.0 GCGGA-TGAGCGGCATTTTCCGT GACGTCTCGTTGCTGCA-TAAACCGACTACACAAATCAGCG ATTTCCATGTTGCC CGCCT ACTCGCCGTAAAAGGCA-CTGCAGAGCAACGACGT ATTTGGCTGATGTGTTTAGTCGC-TAAAGGTACAACGG 71.33 71.39 72.40 73.12 ACTCGC-TTTAATGATGATTTCAGCCGCG CTGTACTGGAGGCTGAAGTT-CAGATGTGCGGCGAGT TGCGTGACTACC TGAGCG AAATTACTACTAAAGTCGGCGC-GACATGACCTCCGACTTCAA GTCTACACGCCGCTCA-ACGCACTGATGG 71.21 70.6 70.93 72.39 TACGGG-TAACAGTTTCTTTATGGCAGGGT GAAACGCAGGTCGCCAG-CGGCACCGCGCCTTTC GGCGGTGAAATTAT ATGCCC ATTGTCAAAGAAATACCGTCCCA-CTTTGCGTCCAGCGGTC GCCGTGGCGCGGAAAG-CCGCCACTTTAATA 71.63 72.51 75.9 73.2 CGATGAGC-GTGGTGGTTATGCCGATCG CGTCACACTACGTCTGAACG-TCGAAAACCCGAAACTGTGG AGCGCCGAA GCTACTCG CACCACCAATACGGCTAGC-GCAGTGTGATGCAGACTTGC AGCTTTTGGGCTTTGACACC-TCGCGGCTT 72.26 71.75 72.28 73.06 ATCCCGA-ATCTCTATCGTGCGGTGGT TGAACTGCACACCGCC-GACGGCACGCTGATTGA AGCAGAAGCCTGCGATG TAGGGCT TAGAGATAGCACGCCACCA-ACTTGACGTGTGGCGG CTGCCGTGCGACTAACT-TCGTCTTCGGACGCTAC 72.20 71.58 71.56 71.42 -TCGGTTTCCGCGAGGT GCGGATTGAAAATGGTCTGC-TGCTGCTGAACGGCAA GCCGTTGCTGATTCGAGG-CGTTA AGCCAAAGGCGCTCCA-CGCCTAACTTTTACCAGACG ACGACGACTTGCCGTT-CGGCAACGACTAAGCTCC GCAAT 72.52 71.49 71.25 72.5 71.34 ACCGTCACGAGCA TCATCCTCTGCATGGTCAG-GTCATGGATGAGCAGACGA TGGTGCAGGATATCCTGC-TGATGAA TGGCAGTGCTCGT-AGTAGGAGACGTACCAGTC CAGTACCTACTCGTCTGCT-ACCACGTCCTATAGGACG ACTACTT 70.53 70.72 70.61 71.22 GCAGAACAACTTTAACG CCGTGCGCTGTTCGCA-TTATCCGAACCATCCGCTG TGGTACACGCTGTGCG-ACCGCTAC CGTCTTGTTGAAATTGC-GGCACGCGACAAGCGT AATAGGCTTGGTAGGCGAC-ACCATGTGCGACACGC TGGCGATG 75.10 71.12 71.1 71.58 GGCCTGTA TGTGGTGGATGAAGCCAA-TATTGAAACCCACGGCATGG TGCCAATGAATCGTCTGACC-GATGATCCGC CCGGACAT-ACACCACCTACTTCGGTT ATAACTTTGGGTGCCGTACC-ACGGTTACTTAGCAGACTGG CTACTAGGCG 70.73 72.34 71.9 72.19 GCTGGC TACCGGCGATGAGCGA-ACGCGTAACGCGAATGG TGCAGCGCGATCGTAA-TCACCCGAGTGTGATCATCT CGACCG-ATGGCCGCTACTCGCT TGCGCATTGCGCTTACC-ACGTCGCGCTAGCATT AGTGGGCTCACACTAGTAGA- 71.90 72.12 70.51 72.12 GGTCGCTGGGGAATGAA-TCAGGCCACGGCGCTA ATCACGACGCGCTGTATC-GCTGGATCAAATCTGTCGATCC TTC CCAGCGACCCCTTACTT AGTCCGGTGCCGCGAT-TAGTGCTGCGCGACATAG CGACCTAGTTTAGACAGCTAGG-AAG 70. 75.43 71.24 72.48 76. CCGCCCGGTGCAG-TATGAAGGCGGCGGAGC CGACACCACGGCCACC-GATATTATTTGCCCGATGTACGC GCGCGTG GGCGGGCCACGTC ATACTTCCGCCGCCTCG-GCTGTGGTGCCGGTGG CTATAATAAACGGGCTACATGCG-CGCGCAC 89 73.70 74. 71.08 72.28 GATGAAGACC-AGCCCTTCCCGGCTGT GCCGAAATGGTCCATCAAAA-AATGGCTTTCGCTACCTGG AGAGACGCGCC CTACTTCTGG TCGGGAAGGGCCGACA-CGGCTTTACCAGGTAGTTTT TTACCGAAAGCGATGGACC-TCTCTGCGCGG 75.41 70.64 71.75 76.57 CGCTG-ATCCTTTGCGAATACGCCC ACGCGATGGGTAACAGTC-TTGGCGGTTTCGCTAAATACT GGCAGGCGTTTCG GCGAC TAGGAAACGCTTATGCGGG-TGCGCTACCCATTGTCAG AACCGCCAAAGCGATTTATGA-CCGTCCGCAAAGC 72.08 70.94 71.86 71.5 TCA-GTATCCCCGTTTACAGGGC GGCTTCGTCTGGGACTG-GGTGGATCAGTCGCTGATTA AATATGATGAAAACGGC AGT CATAGGGGCAAATGTCCCG-CCGAAGCAGACCCTGAC CCACCTAGTCAGCGACTAAT-TTATACTACTTTTGCCG 71.48 70.96 70.80 71.03 AACCC-GTGGTCGGCTTACGGC GGTGATTTTGGCGATACGC-CGAACGATCGCCAGTTCT GTATGAACGGTCTGGTCT TTGGG CACCAGCCGAATGCCG-CCACTAAAACCGCTATGCG GCTTGCTAGCGGTCAAGA-CATACTTGCCAGACCAGA 71.63 70.88 71. 72.79 TTGC-CGACCGCACGCCGCAT CCAGCGCTGACGGAAG-CAAAACACCAGCAGCAGTT TTTCCAGTTCCGTTTATCCGG AACG GCTGGCGTGCGGCGTA-GGTCGCGACTGCCTTC GTTTTGTGGTCGTCGTCAA-AAAGGTCAAGGCAAATAGGCC 77.81 71.60 70.7 71.86 -GCAAACCATCGAAGTGACCAG CGAATACCTGTTCCGTCATAGC-GATAACGAGCTCCTGCACT GGATGGTGGCGCTG CGTTTGGTAGCTTCACTGGTC-GCTTATGGACAAGGCAGTATCG CTATTGCTCGAGGACGTGA-CCTACCACCGCGAC 73.14 72. 70.73 73.90 GAT-GGTAAGCCGCTGGCAAG CGGTGAAGTGCCTCTGG-ATGTCGCTCCACAAGGTAAA CAGTTGATTGAACTGCCTG CTA CCATTCGGCGACCGTTC-GCCACTTCACGGAGACC TACAGCGAGGTGTTCCATTT-GTCAACTAACTTGACGGAC 72.40 71.29 71.00 72.45 AACTA-CCGCAGCCGGAGAGCG CCGGGCAACTCTGGCTC-ACAGTACGCGTAGTGCAACC GAACGCGACCGCATGGT- TTGAT GGCGTCGGCCTCTCGC-GGCCCGTTGAGACCGAG TGTCATGCGCATCACGTTGG-CTTGCGCTGGCGTACCA 77.26 74.32 74.12 74.72 CAGAAGCCGGGCACATC AGCGCCTGGCAGCAGTG-GCGTCTGGCGGAAAACC TCAGTGTGACGCTCCCCG-CCGCGTC GTCTTCGGCCCGTGTAG-TCGCGGACCGTCGTCAC CGCAGACCGCCTTTTGG-AGTCACACTGCGAGGGGC GGCGCAG 72.11 77.49 72.89 75.73 77.12 CCACGCCAT CCCGCATCTGACCACC-AGCGAAATGGATTTTTGCATCG AGCTGGGTAATAAGCGTTGG-CAATTTAAC GGTGCGGTA-GGGCGTAGACTGGTGG TCGCTTTACCTAAAAACGTAGC-TCGACCCATTATTCGCAACC GTTAAATTG 71.11 71.99 71.72 70.5 CGCCAGTCAGG CTTTCTTTCACAGATGTGGATTGG-CGATAAAAAACAACTGCTGACGC CGCTGCGCGATCAGTT-CA GCGGTCAGTCC-GAAAGAAAGTGTCTACACCTAACC GCTATTTTTTGTTGACGACTGCG-GCGACGCGCTAGTCAA GT 71.40 72.43 71.91 75 CCCGTGCACCGCTG GATAACGACATTGGCGTAAGTG-AAGCGACCCGCATTGA CCCTAACGCCTGGGTCG-AACGCTG GGGCACGTGGCGAC-CTATTGCTGTAACCGCATTCAC TTCGCTGGGCGTAACT-GGGATTGCGGACCCAGC TTGCGAC .26 70.81 70.64 73.61 74.77 GAAGGCGGC GGGCCATTACCAGGCC-GAAGCAGCGTTGTTGCAG TGCACGGCAGATACACTT-GCTGATGCGGTGCTG CTTCCGCCG-CCCGGTAATGGTCCGG CTTCGTCGCAACAACGTC-ACGTGCCGTCTATGTGAA CGACTACGCCACGAC 71.38 71.27 70.65 71.37 A TTACGACCGCTCACGC-GTGGCAGCATCAGGGG AAAACCTTATTTATCAGCCGGAAA-ACCTACCGGATTGATGGTA T-AATGCTGGCGAGTGCG CACCGTCGTAGTCCCC-TTTTGGAATAAATAGTCGGCCTTT TGGATGGCCTAACTACCAT 70.75 71.59 70.51 71.95 GT GGTCAAATGGCGATTACCGT-TGATGTTGAAGTGGCGAGC GATACACCGCATCCGGC-GCGGATTGGCCTGAACT CA-CCAGTTTACCGCTAATGGCA ACTACAACTTCACCGCTCG-CTATGTGGCGTAGGCCG CGCCTAACCGGACTTGA- 72.24 72.1 71.80 71.47 GCCAGCTGGCGCAGGTA-GCAGAGCGGGTAAACTGG CTCGGATTAGGGCCGC-AAGAAAACTATCCCGACCGC CTTAC CGGTCGACCGCGTCCAT CGTCTCGCCCATTTGACC-GAGCCTAATCCCGGCG TTCTTTTGATAGGGCTGGCG-GAATG 76.91 71.97 70.83 71.62 70.76 TGCCGCCTGTTTTG-ACCGCTGGGATCTGCC ATTGTCAGACATGTATACCCCG-TACGTCTTCCCGAGCGA AAACGGT ACGGCGGACAAAAC TGGCGACCCTAGACGG-TAACAGTCTGTACATATGGGGC ATGCAGAAGGGCTCGCT-TTTGCCA 73.26 71.18 71.37 72.2 CTGCGCTGC-GGGACGCGCGAATTGA ATTATGGCCCACACCAGTGG-CGCGGCGACTTCCAGTT CAACATCAGCCGCT GACGCGACG CCCTGCGCGCTTAACT-TAATACCGGGTGTGGTCACC GCGCCGCTGAAGGTCAA-GTTGTAGTCGGCGA 71.62 74.1 74.35 72.39 ACAGTC-AACAGCAACTGATGGAAACCAG CCATCGCCATCTGCTGCA-CGCGGAAGAAGGCACAT GGCTGAATATCGA TGTCAG TTGTCGTTGACTACCTTTGGTC-GGTAGCGGTAGACGACGT GCGCCTTCTTCCGTGTA-CCGACTTATAGCT 72.88 74.57 71.68 73.99 CGGTTTCCATA-TGGGGATTGGTGGCGAC GACTCCTGGAGCCCGTC-AGTATCGGCGGAATTCCAGC TGAGCGCCGGT GCCAAAGGTAT ACCCCTAACCACCGCTG-CTGAGGACCTCGGGCAG TCATAGCCGCCTTAAGGTCG-ACTCGCGGCCA 73.77 72.9 73.96 76.2 CGCTAC-CATTACCAGTTGGTCTGGTGTC AAAAATAATAATAAcggctgccgt-ggtagtatagggtgatgggca GCGATG GTAATGGTCAACCAGACCACAG-TTTTTATTATTATTgccgacggca ccatcatatcccactacccgt 72.33 71.03 72.3
Statistics:

Min Tm is 70.0 °C
Avg Tm is 72.33 °C
Max Tm is 77.81 °C
Min hybridization unit size is 16 nucleotides
Avg hybridization unit size is 18.5 nucleotides
Max hybridization unit size is 24 nucleotides

Oligonucleotides to be synthesized:
(All sequence are 5'- 3'. R stand for reverse or (-) strand, F for forward or (+) strand. Sequence in lower case is extra sequence used for termination and does not belong to the input sequence)

R0 GCCAGTGAATCCGTAATCATGGT F0 ACCATGATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTG R23 GCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACG F43 ACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTG R60 CGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAAC F82 CAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCG R100 TCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGG F118 AAGAGGCCCGCACCGATCGCCCTTCCCAACAGT R134 CCATTCAGGCTGCGCAACTGTTGGGAAGGGCGA F151 TGCGCAGCCTGAATGGCGAATGGCGCTTTGCC R167 CTGGTGCCGGAAACCAGGCAAAGCGCCATTCG F183 TGGTTTCCGGCACCAGAAGCGGTGCCGGAAAG R199 GATCGCACTCCAGCCAGCTTTCCGGCACCGCTT F215 CTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGT R232 CCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAA F251 CGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGC R270 TACGTTGGTGTAGATGGGCGCATCGTAACCGTGCATCTG F290 GCCCATCTACACCAACGTAACCTATCCCATTACGGTCAATC R309 CGTGGGAACAAACGGCGGATTGACCGTAATGGGATAGGT F331 CGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTA R348 TCATCAACATTAAATGTGAGCGAGTAACAACCCGTCGGATTCTC F368 CTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGC R392 GCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTT F411 CAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCATCTG R435 AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAAC F456 TGGTGCAACGGGCGCTGGGTCGGTTACGGCCA R472 ACGGCAAACGACTGTCCTGGCCGTAACCGACCC F488 GGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCA R505 TCTCCGGCGCGTAAAAATGCGCTCAGGTCAAATTCAG F525 TTTTTACGCGCCGGAGAAAACCGCCTCGCGGTG R542 TCCAACGCAGCACCATCACCGCGAGGCGGTTT F558 ATGGTGCTGCGTTGGAGTGACGGCAGTTATCTGGA R574 TCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCAC F593 AGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGT R613 TGCAGCAACGAGACGTCACGGAAAATGCCGCTCA F630 GACGTCTCGTTGCTGCATAAACCGACTACACAAATCAGCG R647 GCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTA F670 ATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCG R690 AACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAA F712 CTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGT R732 CCCGTAGGTAGTCACGCAACTCGCCGCACATCTG F748 TGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGT R766 CTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTA F789 GAAACGCAGGTCGCCAGCGGCACCGCGCCTTTC R806 GCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCG F822 GGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCG R844 CGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCAC F863 CGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTGG R883 TCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGA F903 AGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGT R919 GGCGGTGTGCAGTTCAACCACCGCACGATAGAGAT F938 TGAACTGCACACCGCCGACGGCACGCTGATTGA R954 CATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTC F971 AGCAGAAGCCTGCGATGTCGGTTTCCGCGAGGT R988 GCAGACCATTTTCAATCCGCACCTCGCGGAAACCGA F1004 GCGGATTGAAAATGGTCTGCTGCTGCTGAACGGCAA R1024 CCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCA F1040 GCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCA R1058 CTGACCATGCAGAGGATGATGCTCGTGACGGTTAACG F1076 TCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGA R1095 GCAGGATATCCTGCACCATCGTCTGCTCATCCATGAC F1114 TGGTGCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACG R1132 TGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCA F1156 CCGTGCGCTGTTCGCATTATCCGAACCATCCGCTG R1172 CGCACAGCGTGTACCACAGCGGATGGTTCGGATAA F1191 TGGTACACGCTGTGCGACCGCTACGGCCTGTA R1207 TTGGCTTCATCCACCACATACAGGCCGTAGCGGT F1223 TGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGG R1241 GGTCAGACGATTCATTGGCACCATGCCGTGGGTTTCAATA F1261 TGCCAATGAATCGTCTGACCGATGATCCGCGCTGGC R1281 TCGCTCATCGCCGGTAGCCAGCGCGGATCATC F1297 TACCGGCGATGAGCGAACGCGTAACGCGAATGG R1313 TTACGATCGCGCTGCACCATTCGCGTTACGCGT F1330 TGCAGCGCGATCGTAATCACCCGAGTGTGATCATCT R1346 TTCATTCCCCAGCGACCAGATGATCACACTCGGGTGA F1366 GGTCGCTGGGGAATGAATCAGGCCACGGCGCTA R1383 GATACAGCGCGTCGTGATTAGCGCCGTGGCCTGA F1399 ATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATCC R1417 CTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGC F1439 TTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGC R1455 GGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATA F1472 CGACACCACGGCCACCGATATTATTTGCCCGATGTACGC R1488 GGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATC F1511 GCGCGTGGATGAAGACCAGCCCTTCCCGGCTGT R1528 TTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCT F1544 GCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGG R1564 CAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATT F1583 AGAGACGCGCCCGCTGATCCTTTGCGAATACGCCC R1599 GACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGAT F1618 ACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAATACT R1636 TGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAA F1657 GGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGC R1673 CAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATAC F1692 GGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTA R1709 GGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACC F1729 AATATGATGAAAACGGCAACCCGTGGTCGGCTTACGGC R1751 GCGTATCGCCAAAATCACCGCCGTAAGCCGACCAC F1767 GGTGATTTTGGCGATACGCCGAACGATCGCCAGTTCT R1786 GCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCG F1804 GTATGAACGGTCTGGTCTTTGCCGACCGCACGCCGCAT R1826 CTTCCGTCAGCGCTGGATGCGGCGTGCGGTCG F1842 CCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTT R1858 CCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTG F1877 TTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAG R1898 GCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGC F1919 CGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACT R1941 ATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATC F1960 GGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAG R1977 CCAGAGGCACTTCACCGCTTGCCAGCGGCTTACC F1994 CGGTGAAGTGCCTCTGGATGTCGCTCCACAAGGTAAA R2011 TAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACAT F2031 CAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCG R2055 GAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGG F2071 CCGGGCAACTCTGGCTCACAGTACGCGTAGTGCAACC R2088 ACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGT F2108 GAACGCGACCGCATGGTCAGAAGCCGGGCACATC R2125 CACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTG F2142 AGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACC R2159 CGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGC F2176 TCAGTGTGACGCTCCCCGCCGCGTCCCACGCCAT R2194 GGTGGTCAGATGCGGGATGGCGTGGGACGCGG F2210 CCCGCATCTGACCACCAGCGAAATGGATTTTTGCATCG R2226 CCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCT F2248 AGCTGGGTAATAAGCGTTGGCAATTTAACCGCCAGTCAGG R2268 CCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG F2288 CTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTGACGC R2312 AACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCG F2335 CGCTGCGCGATCAGTTCACCCGTGCACCGCTG R2351 CACTTACGCCAATGTCGTTATCCAGCGGTGCACGGGTG F2367 GATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGA R2389 CGACCCAGGCGTTAGGGTCAATGCGGGTCGCTT F2405 CCCTAACGCCTGGGTCGAACGCTGGAAGGCGGC R2422 GGCCTGGTAATGGCCCGCCGCCTTCCAGCGTT F2438 GGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAG R2454 AAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTC F2472 TGCACGGCAGATACACTTGCTGATGCGGTGCTGA R2490 GCGTGAGCGGTCGTAATCAGCACCGCATCAGC F2506 TTACGACCGCTCACGCGTGGCAGCATCAGGGG R2522 TTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCAC F2538 AAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAGT R2562 ACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGT F2583 GGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGC R2603 GCCGGATGCGGTGTATCGCTCGCCACTTCAACATCA F2622 GATACACCGCATCCGGCGCGGATTGGCCTGAACT R2639 TACCTGCGCCAGCTGGCAGTTCAGGCCAATCCGC F2656 GCCAGCTGGCGCAGGTAGCAGAGCGGGTAAACTGG R2673 GCGGCCCTAATCCGAGCCAGTTTACCCGCTCTGC F2691 CTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGC R2707 CAAAACAGGCGGCAGTAAGGCGGTCGGGATAGTTTTCTT F2727 CTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCC R2746 CGGGGTATACATGTCTGACAATGGCAGATCCCAGCGGT F2762 ATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGA R2784 GCAGCGCAGACCGTTTTCGCTCGGGAAGACGTA F2801 AAACGGTCTGCGCTGCGGGACGCGCGAATTGA R2817 CCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCC F2833 ATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTT R2853 GACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCG F2870 CAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAG R2890 TGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTT F2912 CCATCGCCATCTGCTGCACGCGGAAGAAGGCACAT R2930 TATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCG F2947 GGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGAC R2971 GACGGGCTCCAGGAGTCGTCGCCACCAATCCCCA F2988 GACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGC R3005 GTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACT F3025 TGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTC R3042 acggcagccgTTATTATTATTTTTGACACCAGACCAACTGGTAATG F3064 AAAAATAATAATAAcggctgccgtggtagtatagggtgatgggca F3088 tgcccatcaccctatactacc